1. Who is the father of tissue culture?
a) Bonner
b) Haberlandt
c) Laibach
d) Gautheret
Ans – b
2. The production of
secondary metabolites require the use of
a) protoplast
b) cell suspension
c) meristem
d) auxillary buds
Ans - b
3. Synthetic seed is produced
by encapsulating somatic embryo with
a) sodium chloride
b) sodium alginate
c) sodium acetate
d) sodium nitrate
Ans - b
4. Hormone pair required
for a callus to differentiate are
a) auxin and cytokinin
b) auxin and ethylene
c) auxin and absiccic acid
d) cytokinins and
gibberllin
Ans - a
5. DMSO (Dimethyl
sulfoxide) is used as
a) Gelling agent
b) alkaylating agent
c) Chelating agent
d) Cryoprotectant
Ans - d
6. The most widely used
chemical for protoplast fusion, as fusogens, is
a) Manitol
b) Sorbitol
c) Mannol
d) Poly ethylene glycol
(PEG)
Ans – d
7. Cybrids are produced by
a) Fusion of two different
nuclei from two different species
b) Fusion of two same
nuclei from same species
c) Nucleus of one species
but cytoplasm from both the parent species
d) None of the above
Ans – C
8. Callus is
a) Tissue that forms
embryo
b) An insoluble
carbohydrate
c) Tissue that grows to
form embryoid
d) Un organised actively
dividing mass of cells maintained in cultured
Ans - d
9. Part of plant used for
culturing is called
a) Scion
b) Explant
c) Stock
d) Callus
Ans - b
10. Growth hormone
producing apical dominance is
a) Auxin
b) Gibberellin
c) Ethylene
d) Cytokinin
Ans - a
11. A medium which is
composed of chemically defined compound is called
a) Natural media
b) Synthetic media
c) Artificial media
d) None of these
Ans - b
12. To obtain haploid
plant, we culture
a) Entire anther
b) Nucleus
c) Embryo
d) Apical bud
Ans - a
13. Somaclonal variations
are the ones
a) Caused by mutagens
b) Produce during tissue
culture
c) Caused by gamma rays
d) Induced during sexual
embryogeny
Ans - b
14. Which of the following
plant cell will show totipotency?
a) Xylem vessels
b) Sieve tube
c) Meristem
d) Cork cells
Ans - c
15. Which vector is mostly
used in crop improvement?
a) Plasmid
b) Cosmid
c) Phasmid
d) Agrobacterium
Ans - d
16. Plant biotechnology
involves
a) production of valuable
products in plants
b) rapid clonal
multiplication of desired genotypes
c) production of virus
free plants
d) all of these
Ans - d
17. The most common
solidifying agent used in micropropagation is
a) agar
b) dextran
c) Mannan
d) all of these
Ans - a
18. The culturing of cells
in liquid agitated medium is called
a) liquid culture
b) micropropagation
c) Agar culture
d) suspension culture
Ans - d
19. Which of the following
is best suited method for production of virus free plants
a)embryo culture
b) meristem culture
c) ovule culture
d) anther culture
Ans - b
20. Batch cultures are
type of suspension culture where
a) medium is continuously
replaced
b) medium is loaded only
at the beginning
c) no depletion of medium
occurs
d) cellular wastes are
continuously removed and replaced
Ans - b
21. Immobilized cell
bioreactors are based on
a) cells cultures in solid
medium
b) cells cultured in
liquid medium
c) cells entrapped in gels
d) all of these
Ans - c
22. All are plant derived
alkaloids except
a) menthol
b) nicotine
c) quinine
d) codeine
Ans - a
23. Elicitors are
molecules that
a) induce cell divison
b) stimulate production
secondary metabolites
c) stimulate hairy root
formation that accumulate secondary metabolites
d) none of these
Ans - b
24. All are plant derived
elicitors except
a) chitin
b) pectin
c) cellulose
d) pectic acid
Ans - a
25. The modification of
exogenous compounds by plant cells is called
a) Biotransformation
b) bioconversion
c) both a and b
d) biophytomodification
Ans - c
26. Artificial seeds are
a) seeds produced in
laboratory condition
b) seeds encapsulated in a
a gel
c) somatic embryos
encapsulated in a gel
d) zygotic embryos
encapsulated in a gel
Ans - c
27. Hairy root cultures
for secondary metabolite production are induced by transforming plant cells
withs
a) virus
b) Agrobacterium
tumefaciens
c) Bacillus thuringiensis
d) Agrobacterium
rhizogenes
Ans - d
28. The variation in
invitro culture is called as
a) invitro variation
b) mutation
c) somaclonal variation
d) all of these
Ans - c
29. Haploid plants are
produced in large numbers by
a) anther culture
b) Ovary culture
c) both a and b
d) embryo culture
Ans - c
30. Cybrids are
a) nuclear hybrids
b) hybrid plants derived
from cross pollination
c) cytoplasmic hybrids
d) cytological hybrids
Ans - c
31. Golden rice is a
transgenic crop of the future with the following improved trait.
a) Insect resistance
b) High protein content
c) High vitamin A content
d) High lysine content
Ans - c
32. DNA fingerprinting
refer to
a) Techniques used for
identification of fingerprints of individuals
b) Molecular analysis of
profiles of DNA samples
c) Analysis of DNA samples
using imprinting devices
d) Techniques used for
molecular analysis of different specimens of DNA
Ans - b
33. The technique of obtaining large number of
plantlet by tissue culture method is called
a) Plantlet culture
b) Micropropagation
c) Organ culture
d)Macropropagation
Ans - b
34. Maximum
application of animal cell culture technology today is in the production of
a) Insulin
b) Interferons
c) Edible
proteins
d) Vaccines
Ans - d
35. In tissue culture
medium, the embryoids formed from pollen grains is due to
a) Organogenesis
b) Cellular totipotency
c) Double fertilization
d) Test tube culture
Ans - b
36. What is true for
monoclonal antibodies?
a) These antibodies
obtained from one parent and for one antigen
b) These obtained from many
parents and for many antigens
c) These obtained from
different parents and for one antigen
d) These obtained from one
parent and for many antigens
Ans - c
37. Two bacteria found to
be very useful in genetic engineering experiments are
a) Nitrobacter and
Azotobacter
b)Rhizobium and
Diplococcus
c) Nitrosomonas and
Kliebsiella
d) Escherichia and
Agrobacterium
Ans - d
38. A genetically
engineered microorganism used successfully in bioremediation of oil spills is a
species of
a) Trichoderma
b) Bacillus
c) Xanthomonas
d) Pseudomonas
Ans - d
39. Probiotics are
a) cancer inducing
microbes
b) safe antibiotics
c) new kind of food
allergens
d) live microbial food
supplement
Ans - d
40. In order to obtain
virus free plants through tissue culture the best method is
a) meristem culture
b) protoplast culture
c) anther culture
d) embryo rescue
Ans - a
41. A recombinant DNA
molecule is produced by
a) joining of two DNA
fragments
b) joining of two or more
DNA fragments
c) both a and b
d) joining of two or more
DNA fragments originating from different organisms
Ans-d
42. The gene formed by the
joining of DNA segments from two different sources are called as
a) recombinant gene
b) joined gene
c) both a and b
d) chimaeric gene
Ans-d
43. Which of the following
enzyme is used to cut DNA molecule in rDNA technology
a) ligase
b) phosphatase
c) ribonuclease
d) restriction enzymes
Ans-d
44. Restriction enzymes
are also called as
a) biological scissors
b) molecular scalpels
c) molecular knives
d) all of these
Ans-a
45. The most important
discovery that lead to the development of rDNA technology was
a) Double helix model of
Watson and Crick
b) discovery of
restriction enzymes
c) discovery of ligase
enzyme
d) discovery of plasmids
Ans-b
46. Who discovered
restriction enzymes
a) Nathan, Arber and Smith
in 1970
b) Watson, Crick and
Wilkins in 1970
c) Boyer and Cohen in 1975
d) Paul Berg in 1975
Ans-a
47. Who created the first
rDNA molecule
a) Nathan, Arber and Smith
b) Watson, Crick and
Wilkins
c) Boyer and Cohen
d) Paul Berg
Ans-d
48. The DNA molecule to
which the gene of insert is integrated for cloning is called
a) carrier
b) transformer
c) vector
d) none of these
Ans-c
49. The DNA segment to be
cloned is called
a) gene segment
b) DNA fragment
c) DNA insert
d) all of these
Ans-c
50. Which of the following
statements are true regarding rDNA technology
a) rDNA technology is used
to obtain large number of copies of specific DNA fragments
b) rDNA technology is used
to obtain large quantities of the protein produced by the concerned gene
c) rDNA technology is used
to integrate gene of interest into chromosomes where it expresses itself
d) all of these
Ans-d
51. The first successful
transformation of rDNA molecule into a bacterium was carried out by
a) Nathan, Arber and Smith
b) Watson, Crick and
Wilkins
c) Boyer and Cohen
d) Paul Berg.
Ans-c
52. The plasmid used by
Cohen and Boyer for their transformation experiment was
a) pSC 101
b) PUC 17
c) pBR 322
d) E.coli plasmids
Ans-a
53. The mechanism of
intake of DNA fragments from the surrounding medium by a cell is called
a) transformation
b) transduction
c) both a and b
d) conjugation
Ans-a
54. Gene cloning refers to
the
a) production of large number
of copies of the gene being cloned
b) production of asexual
progeny from a single individual or a cell
c) both a and b
d) none of these
Ans-a
55. Paul Berg’s gene
splicing experiment created the first rDNA molecule which was a
a) a T4 phage fragment
incorporated into SV40 vector
b) a lambda phage fragment
incorporated into SV40 vector
c) a T4 phage fragment
incorporated into pSC 101 vector
d) a lambda phage fragment
incorporated into pSC 101 vector
Ans-b
