B.Sc. Part 2 Botany Paper 1 -Molecular Biology and Biotechnology -VIDEO LECTURE



B.Sc. Part 2 Botany Paper 1-  VIDEO LECTURE
जिस टॉपिक का विडियो देखना हो उस टॉपिक पर क्लिक करके आप वीडियो देख सकते है -समस्या होने पर निम्न लिंक पर क्लिक करके भी आप वीडियो देख सकते है - 
https://www.youtube.com/playlist?list=PL4Jtm4XoqYZlTk85dsQD2QaIRnfFD7Qws


PAPER-I

Molecular Biology and Biotechnology

unilt-1

  • Watson and crick model of DNA , 

  •  gene expression
  • Preliminary account of DNA synthesis and repair
  • DNA Replication, 

  • Types of RNA,- m-RNA , r-RNA, t-RNA
  • Genetic code.

Unit-2

  • Central dogma,
  • Reverse transcriptase and its application.
  • Transcription in eukaryotes, -RNA processing capping, splicing and polyadenylation,
  • Translation, initiation, elongation and terminalization
  • Negative and positive control
  • attenuation and antitermination,
  • structure of promoter gene.

Unit-3

  • Biotechnology: Functional definition.
  • Concept of cellular totipotency.
  • Basic aspects of "Plant tissue culture,
  • basal medium,
  • media preparation and
  • aseptic culture technique.
  • Differentiation and morphogenesis.
  • Micro propagation and
  • synthetic Seeds
  • Protoplast culture and
  • somatic hybridization.
  • Anther culture and
  • Androgenic Haploid
  • Embryo culture and application

unit-4

  • Recombinant DNA technology: techniques used in rDNA technology
  • Restriction enzymes.
  • Vectors for gene transfer,
  • Plasmids and cosmids;
  • cDNA technology ,
  • gene amplification
  • Application of Biotechnology and Transgenic Plants
  • Polymerase chain reaction, Application of PCR technique;
  • DNA fingerprinting and its use.

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