BIOLOGY MCQ - BIOTECHNOLOGY FOR BSC MSC

1.  Who is the father of tissue culture?

a) Bonner

b) Haberlandt

c) Laibach

d) Gautheret

Ans – b

2. The production of secondary metabolites require the use of

a) protoplast

b) cell suspension

c) meristem

d) auxillary buds

Ans - b

3. Synthetic seed is produced by encapsulating somatic embryo with

a) sodium chloride

b) sodium alginate

c) sodium acetate

d) sodium nitrate

Ans - b

4. Hormone pair required for a callus to differentiate are

a) auxin and cytokinin

b) auxin and ethylene

c) auxin and absiccic acid

d) cytokinins and gibberllin

Ans - a

5. DMSO (Dimethyl sulfoxide) is used as

a) Gelling agent

b) alkaylating agent

c) Chelating agent

d) Cryoprotectant

Ans - d

6. The most widely used chemical for protoplast fusion, as fusogens, is

a) Manitol

b) Sorbitol

c) Mannol

d) Poly ethylene glycol (PEG)

Ans – d

7. Cybrids are produced by

a) Fusion of two different nuclei from two different species

b) Fusion of two same nuclei from same species

c) Nucleus of one species but cytoplasm from both the parent species

d) None of the above

Ans – C

8. Callus is

a) Tissue that forms embryo

b) An insoluble carbohydrate

c) Tissue that grows to form embryoid

d) Un organised actively dividing mass of cells maintained in cultured

Ans - d

9. Part of plant used for culturing is called

a) Scion

b) Explant

c) Stock

d) Callus

Ans - b

10. Growth hormone producing apical dominance is

a) Auxin

b) Gibberellin

c) Ethylene

d) Cytokinin

Ans - a

11. A medium which is composed of chemically defined compound is called

a) Natural media

b) Synthetic media

c) Artificial media

d) None of these

Ans - b

12. To obtain haploid plant, we culture

a) Entire anther

b) Nucleus

c) Embryo

d) Apical bud

Ans - a

13. Somaclonal variations are the ones

a) Caused by mutagens

b) Produce during tissue culture

c) Caused by gamma rays

d) Induced during sexual embryogeny

Ans - b

14. Which of the following plant cell will show totipotency?

a) Xylem vessels

b) Sieve tube

c) Meristem

d) Cork cells

Ans - c

15. Which vector is mostly used in crop improvement?

a) Plasmid

b) Cosmid

c) Phasmid

d) Agrobacterium

Ans - d

16. Plant biotechnology involves

a) production of valuable products in plants

b) rapid clonal multiplication of desired genotypes

c) production of virus free plants

d) all of these

Ans - d

17. The most common solidifying agent used in micropropagation is

a) agar

b) dextran

c) Mannan

d) all of these

Ans - a

18. The culturing of cells in liquid agitated medium is called

a) liquid culture

b) micropropagation

c) Agar culture

d) suspension culture

Ans - d

19. Which of the following is best suited method for production of virus free plants

a)embryo culture

b) meristem culture

c) ovule culture

d) anther culture

Ans - b

20. Batch cultures are type of suspension culture where

a) medium is continuously replaced

b) medium is loaded only at the beginning

c) no depletion of medium occurs

d) cellular wastes are continuously removed and replaced

Ans - b

21. Immobilized cell bioreactors are based on

a) cells cultures in solid medium

b) cells cultured in liquid medium

c) cells entrapped in gels

d) all of these

Ans - c

22. All are plant derived alkaloids except

a) menthol

b) nicotine

c) quinine

d) codeine

Ans - a

23. Elicitors are molecules that

a) induce cell divison

b) stimulate production secondary metabolites

c) stimulate hairy root formation that accumulate secondary metabolites

d) none of these

Ans - b

24. All are plant derived elicitors except

a) chitin

b) pectin

c) cellulose

d) pectic acid

Ans - a

25. The modification of exogenous compounds by plant cells is called

a) Biotransformation

b) bioconversion

c) both a and b

d) biophytomodification

Ans - c

26. Artificial seeds are

a) seeds produced in laboratory condition

b) seeds encapsulated in a a gel

c) somatic embryos encapsulated in a gel

d) zygotic embryos encapsulated in a gel

Ans - c

27. Hairy root cultures for secondary metabolite production are induced by transforming plant cells withs

a) virus

b) Agrobacterium tumefaciens

c) Bacillus thuringiensis

d) Agrobacterium rhizogenes

Ans - d

28. The variation in invitro culture is called as

a) invitro variation

b) mutation

c) somaclonal variation

d) all of these

Ans - c

29. Haploid plants are produced in large numbers by

a) anther culture

b) Ovary culture

c) both a and b

d) embryo culture

Ans - c

30. Cybrids are

a) nuclear hybrids

b) hybrid plants derived from cross pollination

c) cytoplasmic hybrids

d) cytological hybrids

Ans - c

31. Golden rice is a transgenic crop of the future with the following improved trait.

a) Insect resistance

b) High protein content

c) High vitamin A content

d) High lysine content

  Ans - c

32. DNA fingerprinting refer to

a) Techniques used for identification of fingerprints of individuals

b) Molecular analysis of profiles of DNA samples

c) Analysis of DNA samples using imprinting devices

d) Techniques used for molecular analysis of different specimens of DNA

Ans - b

 33. The technique of obtaining large number of plantlet by tissue culture method is called

a) Plantlet culture

b) Micropropagation

c) Organ culture

d)Macropropagation

Ans - b

34. Maximum application of animal cell culture technology today is in the production of

a) Insulin

b) Interferons

c) Edible proteins

d) Vaccines

Ans - d

 

35. In tissue culture medium, the embryoids formed from pollen grains is due to

a) Organogenesis

b) Cellular totipotency

c) Double fertilization

d) Test tube culture

Ans - b

 

36. What is true for monoclonal antibodies?

a) These antibodies obtained from one parent and for one antigen

b) These obtained from many parents and for many antigens

c) These obtained from different parents and for one antigen

d) These obtained from one parent and for many antigens

Ans - c

 

37. Two bacteria found to be very useful in genetic engineering experiments are

a) Nitrobacter and Azotobacter

b)Rhizobium and Diplococcus

c) Nitrosomonas and Kliebsiella

d) Escherichia and Agrobacterium

Ans - d

 

38. A genetically engineered microorganism used successfully in bioremediation of oil spills is a species of

a) Trichoderma

b) Bacillus

c) Xanthomonas

d) Pseudomonas

Ans - d

 

39. Probiotics are

a) cancer inducing microbes

b) safe antibiotics

c) new kind of food allergens

d) live microbial food supplement

Ans - d

 

40. In order to obtain virus free plants through tissue culture the best method is

a) meristem culture

b) protoplast culture

c) anther culture

d) embryo rescue

Ans - a

41. A recombinant DNA molecule is produced by 

a) joining of two DNA fragments

b) joining of two or more DNA fragments

c) both a and b

d) joining of two or more DNA fragments originating from different organisms

Ans-d

42. The gene formed by the joining of DNA segments from two different sources are called as

a) recombinant gene

b) joined gene

c) both a and b

d) chimaeric gene

Ans-d

43. Which of the following enzyme is used to cut DNA molecule in rDNA technology

a) ligase

b) phosphatase

c) ribonuclease

d) restriction enzymes

Ans-d

44. Restriction enzymes are also called as

a) biological scissors

b) molecular scalpels

c) molecular knives

d) all of these

Ans-a

45. The most important discovery that lead to the development of rDNA technology was

a) Double helix model of Watson and Crick

b) discovery of restriction enzymes

c) discovery of ligase enzyme

d) discovery of plasmids

Ans-b

46. Who discovered restriction enzymes

a) Nathan, Arber and Smith in 1970

b) Watson, Crick and Wilkins in 1970

c) Boyer and Cohen in 1975

d) Paul Berg in 1975

Ans-a

47. Who created the first rDNA molecule

a) Nathan, Arber and Smith

b) Watson, Crick and Wilkins

c) Boyer and Cohen

d) Paul Berg

Ans-d

48. The DNA molecule to which the gene of insert is integrated for cloning is called

a) carrier

b) transformer

c) vector

d) none of these

Ans-c

49. The DNA segment to be cloned is called

a) gene segment

b) DNA fragment

c) DNA insert

d) all of these

Ans-c

50. Which of the following statements are true regarding rDNA technology

a) rDNA technology is used to obtain large number of copies of specific DNA fragments

b) rDNA technology is used to obtain large quantities of the protein produced by the concerned gene

c) rDNA technology is used to integrate gene of interest into chromosomes where it expresses itself

d) all of these

Ans-d

51. The first successful transformation of rDNA molecule into a bacterium was carried out by

a) Nathan, Arber and Smith

b) Watson, Crick and Wilkins

c) Boyer and Cohen

d) Paul Berg.

 Ans-c

52. The plasmid used by Cohen and Boyer for their transformation experiment was

a) pSC 101

b) PUC 17

c) pBR 322

d) E.coli plasmids

Ans-a

53. The mechanism of intake of DNA fragments from the surrounding medium by a cell is called

a) transformation

b) transduction

c) both a and b

d) conjugation

Ans-a

54. Gene cloning refers to the

a) production of large number of copies of the gene being cloned

b) production of asexual progeny from a single individual or a cell

c) both a and b

d) none of these

Ans-a

55. Paul Berg’s gene splicing experiment created the first rDNA molecule which was a

a) a T4 phage fragment incorporated into SV40 vector

b) a lambda phage fragment incorporated into SV40 vector

c) a T4 phage fragment incorporated into pSC 101 vector

d) a lambda phage fragment incorporated into pSC 101 vector

Ans-b